Sex Identification and Development of Single Blastomeres from 2-cell Mouse Embryos.

Effects of Visible Light on the Development of Mouse 2-Cell Embryos

Introduction: Mammalian embryos as well as oocytes are prone to various doses of visible light during manipulations in laboratory. The present study was designed to investigate the effects of visible light on the development of mouse 2- cell embryos. Method: Non-pregnant female NMRI mice were super-ovulated with i.p. injection of 7.5 iu PMSG followed 48 hours later with 10 iu hCG. Forty eight h...

Development of single blastomeres from 4-cell stage embryos after aggregation with parthenogenones in mice.

This study was undertaken to determine whether diploid parthenogenones can assist the development of single blastomeres of 4-cell embryos to term. Isolated blastomeres of 4-cell embryos were aggregated with parthenogenones at the 2-, 4- and 8-cell stages. After the removal of the zonae, aggregation was done by pushing one blastomere of 4-cell embryos and one parthenogenone into contact using a ...

The Effects of Ethanol and Strontium on Growth and Development of Two-Cell Arrested Mouse Embryos

Identical triplets and twins developed from isolated blastomeres of 8- and 16-cell mouse embryos supported with tetraploid blastomeres.

We studied the developmental potential of single blastomeres from early cleavage mouse embryos. Eight- and sixteen-cell diploid mouse embryos were disaggregated and single blastomeres from eight-cell embryos or pairs of sister blastomeres from sixteen-cell embryos were aggregated with 4, 5 or 6 tetraploid blastomeres from 4-cell embryos. Each diploid donor embryo gave eight sister aggregates, w...

I-13: Transcriptome Dynamics of Human and Mouse Preimplantation Embryos Revealed by Single Cell RNA-Sequencing

Background: Mammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. However, it is still unclear about the crucial transcriptional network and key hub genes that regulate the proceeding of preimplantation embryos. Materials and Methods: Through single-cell RNAsequencing (RNA-seq) of both human and mouse preimplantation embryos, ...